Holmes S P, He H, Chen A C, lvie G W, Pietrantonio P V
Department of Entomology, Texas A & M University, College Station, Texas, 77843-2475, USA.
Insect Mol Biol. 2000 Oct;9(5):457-65. doi: 10.1046/j.1365-2583.2000.00208.x.
Leucokinins are invertebrate neuropeptides that exhibit myotropic and diuretic activity. Only one leucokinin-like peptide receptor is known, the lymnokinin receptor from the mollusc Lymnaea stagnalis. A cDNA encoding a leucokinin-like peptide receptor was cloned from the Southern cattle tick, Boophilus microplus, a pest of cattle world-wide. This is the first neuropeptide receptor known from the Acari and the second known in the subfamily of leucokinin-like peptide G-protein-coupled receptors. The deduced amino acid sequence exhibits 40% identity to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative reverse transcription polymerase chain reaction. We also propose that the sequence AAF50775.1 from the Drosophila melanogaster genome (CG10626) encodes the first identified insect leucokinin receptor.
亮氨酸激肽是具有促肌动和利尿活性的无脊椎动物神经肽。目前已知的亮氨酸激肽样肽受体只有一种，即来自软体动物椎实螺的淋巴激肽受体。从全球范围内危害牛群的南方牛蜱（微小牛蜱）中克隆出了编码亮氨酸激肽样肽受体的cDNA。这是蜱螨亚纲已知的首个神经肽受体，也是亮氨酸激肽样肽G蛋白偶联受体亚家族中已知的第二个受体。推导的氨基酸序列与淋巴激肽受体具有40%的同一性。通过半定量逆转录聚合酶链反应确定，该受体转录本存在于蜱的所有生命阶段。我们还提出，果蝇基因组（CG10626）中的序列AAF50775.1编码首个被鉴定的昆虫亮氨酸激肽受体。
