棒络新妇蜘蛛体内沃尔巴克氏体共生菌的超微结构及分子鉴定
Ultrastructural and molecular identification of a Wolbachia endosymbiont in a spider, Nephila clavata.
作者信息
Oh H W, Kim M G, Shin S W, Bae K S, Ahn Y J, Park H Y
机构信息
Insect Resources Laboratory, Korean Collection for Type Cultures, KoreaResearch Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
出版信息
Insect Mol Biol. 2000 Oct;9(5):539-43. doi: 10.1046/j.1365-2583.2000.00218.x.
Wolbachia-like bacteria were observed in the egg cells of golden orb-weaving spider, Nephila clavata, by means of transmission electron microscopy. The bacteria exhibited the typical morphology of Wolbachia, including three enveloping membranes. Based on the amplification and sequencing of partial 16S rDNA and ftsZ gene, the bacteria were identified as Wolbachia, intracellular, transovarially inherited alpha-proteobacteria in invertebrates. Phylogenetic analysis based on 16S rDNA and ftsZ gene sequences invariably indicated that the intracellular bacteria from N. clavata belonged to group A Wolbachia, which were found only from insects. Clustering of Wolbachia from N. clavata with group A Wolbachia indicates that the bacteria were probably transferred horizontally between insects and the spider.
通过透射电子显微镜在金球织网蜘蛛（Nephila clavata）的卵细胞中观察到了类沃尔巴克氏体细菌。这些细菌呈现出沃尔巴克氏体的典型形态，包括三层包膜。基于部分16S rDNA和ftsZ基因的扩增与测序，这些细菌被鉴定为沃尔巴克氏体，是无脊椎动物体内通过卵传递遗传的细胞内α-变形菌。基于16S rDNA和ftsZ基因序列的系统发育分析始终表明，来自N. clavata的细胞内细菌属于A组沃尔巴克氏体，此前仅在昆虫中发现过。来自N. clavata的沃尔巴克氏体与A组沃尔巴克氏体聚类表明，这些细菌可能在昆虫和蜘蛛之间进行了水平转移。
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