Billo P, Marchegiani C, Capella C, Sessa F
Dipartimento di Scienze Cliniche e Biologiche, Università dell'Insubria, Varese.
Pathologica. 2000 Aug;92(4):249-56.
We studied the expression of p53 in 80 gallbladder carcinomas, 43 peritumoral mucosae, 5 adenomas and 20 mucosae of non tumoral gallbladders. Gallbladder cancers were classified according to WHO criteria. We found p53 overexpression in 51 out of the 80 gallbladder cancers (64%). p53 expression was variable in different histologic subtypes: 100% of intestinal type, 66% of papillary type, 83% of adenosquamous carcinomas and 66% of giant cells cancers showed immunoreactive cells. In well and moderately differentiated conventional gallbladder adenocarcinomas we found 60% of positive cases, while, among poorly differentiated conventional cancers, 83% were immunoreactive. All mucinous adenocarcinomas were p53-negative. In peritumoral dysplastic mucosae, p53 was expressed in 23 out 38 cases (60%), 22 (96%) of which were associated to a p53-positive adenocarcinoma. On the contrary, only 5 of the 15 p53-negative dysplastic lesions (33%) were associated to an p53-immunoreactive adenocarcinoma. Metaplastic lesions, of gastric and intestinal type, and adenomas were completely p53-negative. In conclusion, our data suggest that p53 expression is an early event, frequently involved in gallbladder carcinogenesis, and related to different histologic subtypes of gallbladder adenocarcinomas.
我们研究了80例胆囊癌、43例肿瘤周围黏膜、5例腺瘤及20例非肿瘤性胆囊黏膜中p53的表达情况。胆囊癌根据世界卫生组织标准进行分类。我们发现80例胆囊癌中有51例（64%）存在p53过表达。p53在不同组织学亚型中的表达存在差异：肠型中100%、乳头型中66%、腺鳞癌中83%以及巨细胞癌中66%可见免疫反应性细胞。在高分化和中分化的传统胆囊腺癌中，我们发现60%的病例呈阳性，而在低分化的传统胆囊癌中，83%呈免疫反应性。所有黏液腺癌p53均为阴性。在肿瘤周围发育异常的黏膜中，38例中有23例（60%）表达p53，其中22例（96%）与p53阳性的腺癌相关。相反，15例p53阴性的发育异常病变中只有5例（33%）与p53免疫反应性腺癌相关。胃型和肠型化生病变及腺瘤p53均完全为阴性。总之，我们的数据表明p53表达是一个早期事件，频繁参与胆囊癌发生过程，并与胆囊腺癌的不同组织学亚型相关。
