Kehoe C A, Turley E, Bonham M P, O'Connor J M, McKeown A, Faughnan M S, Coulter J S, Gilmore W S, Howard A N, Strain J J
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine, Northern Ireland.
Br J Nutr. 2000 Aug;84(2):151-6.
No sensitive functional index is currently available to assess Cu status in healthy human populations. This study evaluated the effect of Cu supplementation on putative indices of Cu status in twelve women and twelve men, aged between 22 and 45 years, who participated in a double-blind placebo controlled crossover study. The study consisted of three 6-week supplementation regimens of 3 mg CuSO4, 3 mg Cu-glycine chelate and 6 mg Cu-glycine chelate, each separated by placebo periods of equal length. Women had significantly higher caeruloplasmin oxidase activity (P < 0.001), caeruloplasmin protein concentration (P < 0.05), and serum diamine oxidase activity (P < 0.01) at baseline than men. Erythrocyte and leucocyte superoxide dismutase activity, leucocyte cytochrome c oxidase activity, and erythrocyte glutathione peroxidase activity did not respond to Cu supplementation. Platelet cytochrome c oxidase activity was significantly higher (P < 0.01), after supplementation with 6 mg Cu-glycine chelate in the total group and in women but did not change in men. Caeruloplasmin oxidase activity was significantly higher (P < 0.05), in men after supplementation with 3 mg Cu-glycine chelate, while caeruloplasmin protein concentration was significantly lower in men after supplementation with 6 mg Cu-glycine chelate (P < 0.05). Serum diamine oxidase activity was significantly higher after all supplementation regimens in the total group and in both men and women (P < 0.01). These results indicate that serum diamine oxidase activity is sensitive to changes in dietary Cu intakes and may also have the potential to evaluate changes in Cu status in healthy adult human subjects.
目前尚无敏感的功能指标可用于评估健康人群的铜状态。本研究评估了补充铜对12名年龄在22至45岁之间的女性和12名男性铜状态假定指标的影响，这些受试者参与了一项双盲安慰剂对照交叉研究。该研究包括三个为期6周的补充方案，分别为3毫克硫酸铜、3毫克甘氨酸铜螯合物和6毫克甘氨酸铜螯合物，每个方案之间均有等长的安慰剂期。女性在基线时的铜蓝蛋白氧化酶活性（P < 0.001）、铜蓝蛋白蛋白浓度（P < 0.05）和血清二胺氧化酶活性（P < 0.01）显著高于男性。红细胞和白细胞超氧化物歧化酶活性、白细胞细胞色素c氧化酶活性以及红细胞谷胱甘肽过氧化物酶活性对补充铜无反应。在全组和女性中，补充6毫克甘氨酸铜螯合物后血小板细胞色素c氧化酶活性显著升高（P < 0.01），但男性无变化。补充3毫克甘氨酸铜螯合物后男性的铜蓝蛋白氧化酶活性显著升高（P < 0.05），而补充6毫克甘氨酸铜螯合物后男性的铜蓝蛋白蛋白浓度显著降低（P < 0.05）。在全组以及男性和女性中，所有补充方案后血清二胺氧化酶活性均显著升高（P < 0.01）。这些结果表明，血清二胺氧化酶活性对膳食铜摄入量的变化敏感，也可能有潜力评估健康成年受试者铜状态的变化。
