Takahashi Y, Ide T
Laboratory of Nutrition Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Br J Nutr. 2000 Aug;84(2):175-84.
We examined the effect of dietary fats rich in n-3 polyunsaturated fatty acids (PUFA) on mRNA levels in white and brown adipose tissues in rats. Four groups of rats were fed on a low-fat diet (20 g safflower oil/kg) or a high-fat diet (200 g/kg) containing safflower oil, which is rich in n-6 PUFA (linoleic acid), or perilla (alpha-linolenic acid) or fish oil (eicosapentaenoic and docosahexaenoic acids), both of which are rich in n-3 PUFA, for 21 d. Energy intake was higher in rats fed on a high-safflower-oil diet than in those fed on low-fat or high-fish-oil diet, but no other significant differences were detected among the groups. Perirenal white adipose tissue weight was higher and epididymal white adipose tissue weight tended to be higher in rats fed on a high-safflower-oil diet than in those fed on a low-fat diet. However, high-fat diets rich in n-3 PUFA, compared to a low-fat diet, did not increase the white adipose tissue mass. High-fat diets relative to a low-fat diet increased brown adipose tissue uncoupling protein 1 mRNA level. The increases were greater with fats rich in n-3 PUFA than with n-6 PUFA. A high-safflower-oil diet, compared to a low-fat diet, doubled the leptin mRNA level in white adipose tissue. However, high-fat diets rich in n-3 PUFA failed to increase it. Compared to a low-fat diet, high-fat diets down-regulated the glucose transporter 4 mRNA level in white adipose tissue. However, the decreases were attenuated with high-fat diets rich in n-3 PUFA. It is suggested that the alterations in gene expression in adipose tissue contribute to the physiological activities of n-3 PUFA in preventing body fat accumulation and in regulating glucose metabolism in rats.
我们研究了富含n-3多不饱和脂肪酸（PUFA）的膳食脂肪对大鼠白色和棕色脂肪组织中mRNA水平的影响。将四组大鼠分别喂食低脂饮食（20克红花油/千克）或富含n-6 PUFA（亚油酸）的红花油、紫苏（α-亚麻酸）或鱼油（二十碳五烯酸和二十二碳六烯酸，二者均富含n-3 PUFA）的高脂饮食（200克/千克），持续21天。喂食高红花油饮食的大鼠的能量摄入量高于喂食低脂或高鱼油饮食的大鼠，但各组之间未检测到其他显著差异。喂食高红花油饮食的大鼠的肾周白色脂肪组织重量较高，附睾白色脂肪组织重量也有高于喂食低脂饮食大鼠的趋势。然而，与低脂饮食相比，富含n-3 PUFA的高脂饮食并未增加白色脂肪组织的质量。与低脂饮食相比，高脂饮食会增加棕色脂肪组织解偶联蛋白1的mRNA水平。富含n-3 PUFA的脂肪所引起的增加幅度大于富含n-6 PUFA的脂肪。与低脂饮食相比，高红花油饮食使白色脂肪组织中的瘦素mRNA水平增加了一倍。然而，富含n-3 PUFA的高脂饮食未能使其增加。与低脂饮食相比，高脂饮食会下调白色脂肪组织中葡萄糖转运蛋白4的mRNA水平。然而，富含n-3 PUFA的高脂饮食会减弱这种下降。提示脂肪组织中基因表达的改变有助于n-3 PUFA在预防大鼠体脂积累和调节葡萄糖代谢方面的生理活性。
