Odintsova M S, Yurina N P
Orig Life. 1975 Jul;6(3):441-6. doi: 10.1007/BF01130347.
The buoyant density in CsCl of ribosomes from chloroplasts of the green alga Chlorella pyrenoidosa and two species of higher plants, Pisum sativum and Chenopodium album, has been studied. From the relative protein content it was calculated that 70S ribosomes from chloroplasts are much smaller than 80S cytoplasmic ribosomes (3.0-3.1 X 10(6) and 4.0 X 10(6) daltons) and slightly larger than 70S ribosomes from bacteria (E. coli 2.5 X 10(6) daltons). Chloroplast ribosomes from pea seedlings were analyzed by two-dimensional polyacrylamide gel electrophoresis. They appear to contain 71 proteins. This indicates that chloroplast ribosomes contain a larger number of proteins than do the ribosomes from E. coli and other species of Enterobacteriaceae. Further study will permit a probable evaluation of the validity of Mereschkowsky's hypothesis that the photosynthetic plastids of eukaryotic plant cells are the evolutionary descendants of endosymbiotic blue-green algae.
对绿藻小球藻以及两种高等植物豌豆和藜的叶绿体核糖体在氯化铯中的浮力密度进行了研究。根据相对蛋白质含量计算得出，叶绿体中的70S核糖体比80S细胞质核糖体（3.0 - 3.1×10⁶和4.0×10⁶道尔顿）小得多，比细菌（大肠杆菌2.5×10⁶道尔顿）中的70S核糖体稍大。通过二维聚丙烯酰胺凝胶电泳分析了豌豆幼苗的叶绿体核糖体。它们似乎含有71种蛋白质。这表明叶绿体核糖体比大肠杆菌和其他肠杆菌科物种的核糖体含有更多的蛋白质。进一步的研究将有可能评估梅列日科夫斯基假说的正确性，该假说认为真核植物细胞的光合质体是内共生蓝藻的进化后代。
