酿酒酵母线粒体DNA的异质性及tRNA的遗传信息。
Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.
作者信息
Baldacci G, Carnevali F, Frontali L, Leoni L, Macino G, Palleschi C
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1777-86. doi: 10.1093/nar/2.10.1777.
Abstract
Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while only genes for tRNAser and tRNAhis are present on petite mitochondrial DNA; moreover the hybridization patterns indicate that these genes are not clustered and suggest that more than one gene might exist for tRNAser and tRNAhis.
摘要
通过对经超声处理并在氯化铯梯度上分级分离得到的不同浮力密度的DNA片段上的几种tRNA进行杂交，分析了野生型酿酒酵母和一个“极端”小菌落突变体的线粒体DNA。杂交模式表明，tRNAser、tRNAphe、tRNAhis、tRNAval、tRNAileu的基因存在于野生型线粒体DNA上，而小菌落线粒体DNA上仅存在tRNAser和tRNAhis的基因；此外，杂交模式表明这些基因并非成簇存在，并且提示tRNAser和tRNAhis可能存在不止一个基因。
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