[umuC 突变对紫外线和γ射线照射作用下大肠杆菌细胞中 SOS 应答诱导的影响]

[The effect of am umuC mutation on the induction of an SOS response in E. coli cella under the action of UV and gamma irradiation].

作者信息

Komova O V, Kandiano E S, Krasavin E A

机构信息

Joint Institute for Nuclear Research, Dubna, Moscow oblast, Russia.

出版信息

Radiats Biol Radioecol. 2000 Jul-Aug;40(4):378-81.

PMID:11031480

Abstract

The kinetics of the SOS induction in E. coli cells of wild type and deficient in umuC gene exposed to UV and gamma-rays were analysed. In the presence of UmuC protein SOS induction was 3-5.5 times lower and delayed for about 30 minutes after both UV and gamma rays. It was shown that decrease of the SOS induction in wild type cells irradiated by UV was due to more effective elimination of the photolesions from DNA by excision repair system. UmuCD-dependent inhibition of DNA replication was discussed as a possible mechanism allowing additional time for error-free repair.

摘要

分析了野生型和缺乏umuC基因的大肠杆菌细胞在暴露于紫外线和γ射线时SOS诱导的动力学。在存在UmuC蛋白的情况下，紫外线和γ射线照射后SOS诱导降低3 - 5.5倍且延迟约30分钟。结果表明，紫外线照射的野生型细胞中SOS诱导的降低是由于切除修复系统更有效地从DNA中消除了光损伤。讨论了UmuCD依赖性的DNA复制抑制作为允许额外时间进行无差错修复的一种可能机制。