Fresneau C, Zinovieva M, Blanot D, Batelier G, Arrio B
Laboratoire de Bioénergétique Membranaire, A.N. Bakh Institute of Biochemistry, Russian Academy of Science, Moscow.
IUBMB Life. 2000 Jun;49(6):527-32. doi: 10.1080/15216540050167070.
Expression of a 126-kDa protein in the plasmalemma (cytoplasmic membrane) from Synechococcus PCC 7942 is dependent on the nitrogen source. Polyclonal antibody raised against the NrtA protein reacted with the 126-kDa protein. Two peptide sequences from the 126-kDa protein were retrieved in NrtA. FPLC purification of plasmalemma solubilised in Triton X-100 gave a fraction consisting mainly of a 126-kDa component (72.6%), as shown by sedimentation velocity. Equilibrium sedimentation of the same fraction gave evidence of the existence of an oligomeric structure (Ka = 2 x 10(3) and 2.9 subunits). Thus, the 126-kDa protein is considered as a trimeric arrangement of the 45-kDa protein in the plasmalemma.
聚球藻PCC 7942质膜（细胞质膜）中一种126 kDa蛋白质的表达取决于氮源。针对NrtA蛋白产生的多克隆抗体与该126 kDa蛋白质发生反应。在NrtA中检索到了来自126 kDa蛋白质的两个肽序列。用Triton X - 100溶解质膜后进行快速蛋白质液相色谱（FPLC）纯化，得到一个主要由126 kDa组分（72.6%）组成的级分，沉降速度分析表明了这一点。对同一级分进行平衡沉降证明存在寡聚结构（Ka = 2×10³，2.9个亚基）。因此，126 kDa蛋白质被认为是质膜中45 kDa蛋白质的三聚体排列。
