Díez B, Antón J, Guixa-Boixereu N, Pedrós-Alió C, Rodríguez-Valera F
Department of Marine Biology and Oceanography, Institute of Marine Sciences, CSIC, Barcelona, Spain.
Int Microbiol. 2000 Sep;3(3):159-64.
A method for analyzing virus assemblages in aquatic environments was developed and used for studying the highest-salinity ponds (from 13.4 to 35% salinity) from a multi-pond solar saltern in Alicante, Spain. The protocol consisted of a series of concentration and purification steps including tangential flow filtration and ultracentrifugation, followed by the preparation of total viral nucleic acids that were subsequently separated by pulsed-field gel electrophoresis. For every sample analyzed, a characteristic DNA pattern was obtained, whose complexity was related to viral diversity. The comparison of our results with a similar analysis carried out with marine virus assemblages shows that, as expected, the viral diversity corresponding to the analyzed hypersaline environment is considerably lower than that of a marine environment.
开发了一种分析水生环境中病毒群落的方法，并将其用于研究西班牙阿利坎特一个多池塘太阳能盐场中盐度最高的池塘（盐度从13.4%到35%）。该方案包括一系列浓缩和纯化步骤，包括切向流过滤和超速离心，随后制备总病毒核酸，然后通过脉冲场凝胶电泳进行分离。对于每个分析的样本，都获得了一种特征性的DNA图谱，其复杂性与病毒多样性相关。将我们的结果与对海洋病毒群落进行的类似分析进行比较表明，正如预期的那样，与所分析的高盐环境相对应的病毒多样性明显低于海洋环境。
