Nijhof M W, Dhert W J, Fleer A, Vogely H C, Verbout A J
University Cluster of Orthopaedics, University Medical Center Utrecht, G05.228, P.O. Box 85500, NL 3508 GA Utrecht, The Netherlands.
J Biomed Mater Res. 2000 Dec 15;52(4):754-61. doi: 10.1002/1097-4636(20001215)52:4<754::aid-jbm21>3.0.co;2-#.
In a rabbit model, premixed tobramycin-containing bone cement was studied for its efficacy to prevent infections with two frequently encountered staphylococcal species in arthroplasty surgery. After intramedullary inoculation with staphylococci, either standard or premixed tobramycin-containing Simplex-P bone cement was injected in the right femur of 120 rabbits. Development of infection was examined by culture of femoral bone after 7 or 28 days. Loss of body weight and elevated erythrocyte sedimentation rate in the control rabbits inoculated with Staphylococcus aureus were seen in the first postoperative week, returning to normal in 28 days. Inoculation with Staphylococcus epidermidis resulted only in a low-grade infection. All rabbits receiving premixed tobramycin-containing bone cement were free of signs of infection, and all their cultures were negative. Culture yield from Staphylococcus aureus controls increased with time and inoculum dose. Staphylococcus epidermidis controls needed higher inoculum doses to establish an infection, while culture yield decreased in time. These differences in mode of prosthesis-related infection are explained by differences in virulence factors.
在兔模型中，研究了含预混妥布霉素的骨水泥预防关节置换手术中两种常见葡萄球菌感染的效果。在对兔子进行髓内接种葡萄球菌后，将标准的或含预混妥布霉素的Simplex-P骨水泥注入120只兔子的右侧股骨。7天或28天后通过股骨培养检查感染的发生情况。接种金黄色葡萄球菌的对照兔在术后第一周出现体重减轻和红细胞沉降率升高，28天后恢复正常。接种表皮葡萄球菌仅导致轻度感染。所有接受含预混妥布霉素骨水泥的兔子均无感染迹象，且所有培养结果均为阴性。金黄色葡萄球菌对照组的培养产量随时间和接种剂量增加。表皮葡萄球菌对照组需要更高的接种剂量来引发感染，而培养产量随时间下降。假体相关感染模式的这些差异可由毒力因子的差异来解释。
