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[酿酒酵母菌株中的隐蔽异核体。检测隐蔽细胞核的条件]

[Cryptic heterokaryons in strains of Saccharomyces cerevisiae. Conditions for detecting a cryptic nucleus].

作者信息

Nevzgliadova O V, Smirnova T I, Gaĭvoronskiĭ A A, Soĭdla T R

机构信息

Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.

出版信息

Genetika. 2000 Aug;36(8):1017-24.

Abstract

A phenomenon discovered earlier, cryptic heterokaryosis in Saccharomyces yeast, has been further investigated. A phenotypically silent nucleus in a yeast cell may resume its expression after fusion with another parental cell. The resulting hybrid is capable of sporulation. By the growth of a cytoductant with an expressing nucleus from one parent and a silent nucleus from the other parent on suitable selective media, the silent nucleus can be activated. The presence of deletion or insertion mutations in several genes in YPH strains allows nuclei of the YPH type to be traced not only genetically but also by blotting.

摘要

一种先前发现的现象,即酿酒酵母中的隐蔽异核现象,已得到进一步研究。酵母细胞中一个表型沉默的细胞核在与另一个亲代细胞融合后可能会恢复其表达。产生的杂种能够形成孢子。通过将具有来自一个亲本的表达细胞核和来自另一个亲本的沉默细胞核的细胞导管体在合适的选择培养基上培养,沉默的细胞核可以被激活。YPH菌株中几个基因存在缺失或插入突变,这使得不仅可以通过遗传学方法,还可以通过印迹法追踪YPH型细胞核。

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