Khusseĭn I A, Kochneva E Z, Khadeeva N V
Timiryazev Agricultural Academy, Russian Academy of Sciences, Moscow, Russia.
Genetika. 2000 Aug;36(8):1093-9.
Nodular segments of the stem and stolons of aseptic Stachys sieboldii (Miq.) plants were cultured in Gamborg's B5 medium at a low (1-2 mg/l) or high (10 mg/l) concentration of cytokinins (6-BAP, kinetin) and produced many adventitious buds and shoots without callus formation. Several plants were regenerated from nodular segments incubated at various concentrations of 5-bromodeoxiuridine (5-BdU) for three weeks. All regenerants were morphologically normal. Peroxidase electrophoretic patterns were analyzed to determine whether somaclonal variation is associated with S. sieboldii micropropagation. The frequency of changed forms was higher with the high 6-BAP concentration and with mutagenic treatment. Changed forms were not detected in plants grown at a low cytokinin concentration, which can be used in S. sieboldii micropropagation.
将无菌栽培的绵枣儿（Stachys sieboldii (Miq.)）植株的茎和匍匐茎的结节段在含有低浓度（1-2毫克/升）或高浓度（10毫克/升）细胞分裂素（6-苄基腺嘌呤、激动素）的甘博格B5培养基中培养，可产生许多不定芽和芽苗，且不形成愈伤组织。将结节段在不同浓度的5-溴脱氧尿苷（5-BdU）中培养三周，再生出了几株植株。所有再生植株形态均正常。分析过氧化物酶电泳图谱以确定体细胞无性系变异是否与绵枣儿的微繁殖有关。6-苄基腺嘌呤高浓度处理和诱变处理时，变异形式的频率更高。在低细胞分裂素浓度下生长的植株中未检测到变异形式，这些植株可用于绵枣儿的微繁殖。
