Mironova A P, Rozanov Iu M
Institute of Cytology RAS, St. Petersburg.
Tsitologiia. 2000;42(8):725-32.
Effect of acclimating temperature on the thermoresistance of isolated ciliated gill epithelium of Anodonta anatina was studied. One strip of gills from each mussel was used to determine the initial level of thermoresistance (survival time at 40 degrees C). Other strips were kept at 24 degrees C until their death, and their thermoresistance and fluorescence after Ethidium bromide staining were determined at different time intervals. After a short exposure at 24 degrees C the mean level of thermoresistance of the epithelium increased. The individual shift in the value and direction of cell thermoresistance in different mussels negatively correlated with its initial level. It resulted in narrowing the extent of individual variability of the level of thermoresistance. The prolongation of exposure at 24 degrees C decreased the mean level of thermoresistance, disturbed the negative correlation, and increased the variability of thermoresistance level. The value of cell fluorescence remained constant within 48 h. The increase in fluorescence was observed 12 h after reducing the mean thermoresistance level. Hence, the dynamics of variability in the thermoresistance level is a most sensitive indication of the cell functional state.
研究了驯化温度对无齿蚌离体纤毛鳃上皮耐热性的影响。从每个贻贝上取下一条鳃来确定耐热性的初始水平（在40摄氏度下的存活时间）。其他鳃条在24摄氏度下保存直至死亡，并在不同时间间隔测定其耐热性和溴化乙锭染色后的荧光。在24摄氏度下短暂暴露后，上皮的平均耐热性水平增加。不同贻贝中细胞耐热性值和方向的个体变化与其初始水平呈负相关。这导致耐热性水平个体变异性的范围缩小。在24摄氏度下延长暴露时间会降低平均耐热性水平，扰乱负相关，并增加耐热性水平的变异性。细胞荧光值在48小时内保持恒定。在平均耐热性水平降低12小时后观察到荧光增加。因此，耐热性水平变异性的动态是细胞功能状态最敏感的指标。
