Ginzinger D G, Godfrey T E, Nigro J, Moore D H, Suzuki S, Pallavicini M G, Gray J W, Jensen R H
Department of Laboratory Medicine, University of California San Francisco Comprehensive Cancer Center, University of California, 94143, USA.
Cancer Res. 2000 Oct 1;60(19):5405-9.
This report describes the development and validation of quantitative microsatellite analysis (QuMA) for rapid measurement of relative DNA sequence copy number. In QuMA, the copy number of a test locus relative to a pooled reference is assessed using quantitative, real-time PCR amplification of loci carrying simple sequence repeats. Use of simple sequence repeats is advantageous because of the large numbers that are mapped precisely. In addition, all markers are informative because QuMA does not require that they be polymorphic. The utility of QuMA is demonstrated in assessment of the extent of deletions of chromosome 2 in leukemias arising in radiation-sensitive inbred SJL mice and in analysis of the association of increased copy number of the putative oncogene ZNF217 with reduced survival duration in ovarian cancer patients.
本报告描述了用于快速测量相对DNA序列拷贝数的定量微卫星分析（QuMA）的开发与验证。在QuMA中，通过对携带简单序列重复的位点进行定量实时PCR扩增，评估测试位点相对于混合参考的拷贝数。使用简单序列重复具有优势，因为有大量精确映射的重复序列。此外，所有标记都是信息丰富的，因为QuMA不要求它们具有多态性。QuMA的实用性在评估辐射敏感近交系SJL小鼠白血病中2号染色体缺失程度以及分析卵巢癌患者中假定癌基因ZNF217拷贝数增加与生存期缩短的关联中得到了证明。
