Mukai S T, Almeida K, Hatiras G
Department of Biology, York University, Toronto, Ontario, Canada.
Acta Biol Hung. 2000;51(2-4):243-53.
The albumen gland in Helisoma secretes a perivitelline fluid which surrounds each egg and is made up of several 66 kDa protein subunits and polysaccharide complexes. Forskolin, an adenylate cyclase activator, stimulated the secretion and release of the perivitelline fluid. An acidic extract of the central nervous system increased the intracellular concentration of cAMP in the albumen gland and this results in the release of the 66 kDa molecule and other proteins. Digestion of the brain extract with proteases abolished this activity, suggesting that the factor is a peptide. Cyclic AMP analogues and [BMX also stimulated the protein secretion in dose-dependent manner. Forskolin when added with the brain factor had an additive response. SQ22536, a non-competitive inhibitor of adenylate cyclase, inhibited brain extract dependent adenylate cyclase activity whereas aluminum fluoride, a G protein activator, was found to stimulate adenylate cyclase. Dopamine also stimulates protein secretion by the albumen gland and through the application of various agonists and antagonists of dopamine, it was established that the neurotransmitter acts via D1-like receptors by stimulating adenylate cyclase.
椎实螺的蛋白腺分泌一种围绕每个卵的卵周液，它由几个66 kDa的蛋白质亚基和多糖复合物组成。福斯高林，一种腺苷酸环化酶激活剂，刺激了卵周液的分泌和释放。中枢神经系统的酸性提取物增加了蛋白腺中细胞内cAMP的浓度，这导致了66 kDa分子和其他蛋白质的释放。用蛋白酶消化脑提取物消除了这种活性，表明该因子是一种肽。环磷酸腺苷类似物和[BMX也以剂量依赖的方式刺激蛋白质分泌。福斯高林与脑因子一起添加时具有相加反应。SQ22536，一种腺苷酸环化酶的非竞争性抑制剂，抑制脑提取物依赖性腺苷酸环化酶活性，而氟化铝，一种G蛋白激活剂，被发现可刺激腺苷酸环化酶。多巴胺也刺激蛋白腺的蛋白质分泌，通过应用多巴胺的各种激动剂和拮抗剂，确定该神经递质通过刺激腺苷酸环化酶经由D1样受体起作用。
