Lahmy S, Barnèche F, Derancourt J, Filipowicz W, Delseny M, Echeverria M
Laboratoire Génome et Développement des Plantes', Université de Perpignan UMR CNRS 5096, France.
FEBS Lett. 2000 Sep 1;480(2-3):255-60. doi: 10.1016/s0014-5793(00)01935-9.
P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalisation experiments in transfected protoplasts demonstrated that this protein is addressed to the chloroplast. The RNA-binding activity of recombinant P67 was found to be similar to that of the native protein. A significant similarity with the maize protein CRP1 [Fisk, D.G., Walker, M.B. and Barkan, A. (1999) EMBO J. 18, 2621-2630] suggests that P67 belongs to the PPR family and could be involved in chloroplast RNA processing.
P67是一种与特定RNA探针结合的新蛋白质，从萝卜幼苗中纯化得到[埃切维里亚，M.和拉米，S.（1995年）《核酸研究》23卷，4963 - 4970页]。从P67微量测序获得的氨基酸序列信息使得能够在萝卜和拟南芥中分离出编码P67的基因。在转染原生质体中的免疫定位实验表明该蛋白质定位于叶绿体。发现重组P67的RNA结合活性与天然蛋白质相似。与玉米蛋白质CRP1[菲斯克，D.G.，沃克，M.B.和巴尔坎，A.（1999年）《欧洲分子生物学组织杂志》18卷，2621 - 2630页]的显著相似性表明P67属于PPR家族，可能参与叶绿体RNA加工。
