Xiao H, Harvey K, Labarrere C A, Kovacs R
Methodist Research Institute, 1701 N. Senate Boulevard, Indianapolis, Indiana 46206-1367, USA.
Cryobiology. 2000 Sep;41(2):97-105. doi: 10.1006/cryo.2000.2271.
Current methods of platelet storage are unsatisfactory because of the short shelf life of platelets and the rapid loss of platelet viability. We have developed a cryopreservation method that results in less damage from freezing and higher recovered function of platelets. Platelets were cryopreserved using a combination of epinephrine (EPN) and dimethyl sulfoxide (Me(2)SO) as cryoprotectants. The response of platelets to agonists was studied by flow cytometry and aggregation tests. Cryopreserving platelets with Me(2)SO decreased platelet annexin V binding due to freezing. The combination of EPN with Me(2)SO enhanced Me(2)SO cryoprotection and decreased platelet microparticle generation, suggesting that cryopreserving platelets using this combination is associated with increased platelet integrity. Platelet cryopreservation with an Me(2)SO/EPN combination also increased platelet aggregability, which was demonstrated by decreasing the lag phase and increasing the aggregation density to 66.39% +/- 6.6 that of fresh platelet-rich plasmas. We conclude that adding EPN as a combined cryoprotectant improves the quality of Me(2)SO-frozen platelets. As a method of aggregation of cryopreserved platelets, this method is comparable to that of normal fresh platelets and may improve the conditions for platelet transfusion.
目前的血小板储存方法并不理想，因为血小板的保质期短且血小板活力迅速丧失。我们开发了一种冷冻保存方法，该方法可减少冷冻损伤并提高血小板的恢复功能。使用肾上腺素（EPN）和二甲基亚砜（Me(2)SO）的组合作为冷冻保护剂对血小板进行冷冻保存。通过流式细胞术和聚集试验研究血小板对激动剂的反应。用Me(2)SO冷冻保存血小板会因冷冻而降低血小板膜联蛋白V结合。EPN与Me(2)SO的组合增强了Me(2)SO的冷冻保护作用并减少了血小板微粒的产生，这表明使用这种组合冷冻保存血小板与血小板完整性增加有关。用Me(2)SO/EPN组合冷冻保存血小板也增加了血小板聚集性，这通过缩短延迟期和将聚集密度提高到新鲜富血小板血浆的66.39%±6.6%得到证明。我们得出结论，添加EPN作为联合冷冻保护剂可提高Me(2)SO冷冻血小板的质量。作为冷冻保存血小板的一种聚集方法，该方法与正常新鲜血小板的方法相当，可能会改善血小板输血的条件。
