Nowak J, Horst A
Ann Med Sect Pol Acad Sci. 1975;20(3):173-84.
Employing the surface immunofluorescence technique and rosette test, surface receptors of lymphocytes from 83 healthy subjects (57 adult donors and 2l infants), 3 mature fetuses, and 110 patients, including 23 infants with acute repiratory infections, 22 patients with multiple sclerosis (MS), 20 patients with viral hepatitis (VH), 22 patients with chronic brucellosis, and 1 patient with ataxia-telangiectasia lacking serum IgA were studied. Surface immunoglobulins complement-receptor lymphocyte (B lymphocytes), rosette formation with uncoated sheep erythrocytes (T lymphocytes), and receptor for Fc IgG, characteristic mainly of B lymphocytes, were determined. Percentages of B lymphocytes in peripheral blood of adults (21%) and infants (about 24%), T lymphocytes in adults (about 70%) and infants (about 52%) were determined. On the surface of lymphocytes from adults, IgM predominated, followed by IgG, and IgA was least frequent. In acute upper respiratory tract infections in infants percentages of B lymphocytes in peripheral blood were markedly increased. Glucocorticoids used in treatment exerted a distinctly suppressive effect on these cells. In multiple sclerosis the number of cells with receptors for complement and density of receptors for Fc IgG on the surface of lymphocytes were decreased. In acute viral hepatitis, no significant changes in the contents of B and T lymphocytes in peripheral blood were noted. In chronic brucellosis, the numbers of T lymphocytes were decreased, and atypical mononuclear cells with partial lack of receptors typical of B lymphocytes were observed. The findings indicate that in diseases based on bacterial or viral infections and in multiple sclerosis, B and T lymphocytes play an essential role, which is reflected by the percentages of B and T lymphocytes in peripheral blood.
采用表面免疫荧光技术和玫瑰花结试验,对83名健康受试者(57名成年供血者和21名婴儿)、3例成熟胎儿以及110例患者的淋巴细胞表面受体进行了研究,其中患者包括23例急性呼吸道感染婴儿、22例多发性硬化症(MS)患者、20例病毒性肝炎(VH)患者、22例慢性布鲁氏菌病患者以及1例缺乏血清IgA的共济失调-毛细血管扩张症患者。测定了表面免疫球蛋白补体受体淋巴细胞(B淋巴细胞)、与未包被绵羊红细胞形成玫瑰花结的情况(T淋巴细胞)以及主要为B淋巴细胞特征性的Fc IgG受体。确定了成年人外周血中B淋巴细胞的百分比(21%)和婴儿外周血中B淋巴细胞的百分比(约24%),成年人外周血中T淋巴细胞的百分比(约70%)和婴儿外周血中T淋巴细胞的百分比(约52%)。在成年人淋巴细胞表面,IgM占主导,其次是IgG,IgA最少见。婴儿急性上呼吸道感染时,外周血中B淋巴细胞百分比明显增加。治疗中使用的糖皮质激素对这些细胞有明显的抑制作用。在多发性硬化症中,淋巴细胞表面补体受体细胞数量和Fc IgG受体密度降低。在急性病毒性肝炎中,外周血中B和T淋巴细胞含量未发现明显变化。在慢性布鲁氏菌病中,T淋巴细胞数量减少,观察到部分缺乏典型B淋巴细胞受体的非典型单核细胞。研究结果表明,在基于细菌或病毒感染的疾病以及多发性硬化症中,B和T淋巴细胞发挥着重要作用,这在外周血中B和T淋巴细胞的百分比中得到体现。