Gossmann A, Helbich T H, Mesiano S, Shames D M, Wendland M F, Roberts T P, Ferrara N, Jaffe R B, Brasch R C
Center for Pharmaceutical and Molecular Imaging, Department of Radiology, University of California San Francisco, San Francisco, CA 94143-0628, USA.
Am J Obstet Gynecol. 2000 Oct;183(4):956-63. doi: 10.1067/mob.2000.107092.
Magnetic resonance imaging enhanced with macromolecular contrast medium was used to monitor effects of angiogenesis inhibition on tumor microvascular permeability and ascites volume in an athymic rat model of human ovarian cancer.
Groups of 6 athymic rats implanted intraperitoneally with SKOV-3, a human ovarian cancer cell line, were treated through a 14-day course with antibody to vascular endothelial growth factor or with saline solution for control animals. Dynamic magnetic resonance imaging was performed with a 92,000-d contrast agent, albumin-(gadolinium-diethylenetriaminepentaacetic acid)(30). Vascular permeability was estimated from dynamic enhancement data that were analyzed with a unidirectional 2-compartment kinetic model.
Animals treated with vascular endothelial growth factor antibody accumulated significantly smaller volumes of peritoneal ascites (P <.05) and showed significantly lower magnetic resonance imaging-assayed tumor microvascular permeabilities (P <.05) than did control animals.
Magnetic resonance imaging enhanced with a macromolecular contrast agent in an athymic rat model of human ovarian cancer treated with anti-vascular endothelial growth factor antibody can be used to measure a reduction in tumor microvascular permeability, corresponding to a reduction in ascites production.
在人卵巢癌裸鼠模型中，采用大分子对比剂增强磁共振成像来监测血管生成抑制对肿瘤微血管通透性和腹水量的影响。
将6组腹腔内植入人卵巢癌细胞系SKOV-3的裸鼠，用血管内皮生长因子抗体治疗14天，对照组动物用生理盐水治疗。使用92000道尔顿的对比剂白蛋白-（钆-二乙烯三胺五乙酸）（30）进行动态磁共振成像。根据用单向双室动力学模型分析的动态增强数据估算血管通透性。
与对照动物相比，用血管内皮生长因子抗体治疗的动物腹腔内腹水积聚量显著较小（P<.05），磁共振成像测定的肿瘤微血管通透性显著较低（P<.05）。
在用抗血管内皮生长因子抗体治疗的人卵巢癌裸鼠模型中，大分子对比剂增强的磁共振成像可用于测量肿瘤微血管通透性的降低，这与腹水产生的减少相对应。
