Wold E, Brustad T
Acta Chem Scand B. 1975;29(7):781-6. doi: 10.3891/acta.chem.scand.29b-0781.
Binding of tritiated 2,2,6,6-tetramethyl-4-piperidone-N-oxyl (3H-TAN) to radiation-induced DNA-transients in E. coli K-12 strains AB 1157 and JO 307 rec A uvr A has been studied under in vivo conditions. After irradiation the cells were washed and resuspended in growth medium and left overnight at 37 degrees C. Within an uncertainty of about 10%, no effect of repair could be detected on the yield of TAN bound to DNA for any of the strains. During the period after resuspension. TAN or fragments of TAN leaked out of the irradiated cell samples. This leakage may be attributed to semi-permanant association between TAN and radiation-induced radicals within the cell. The relevance of different interactions between TAN and transients in DNA is discussed.
在体内条件下，研究了氚化的2,2,6,6-四甲基-4-哌啶酮-N-氧基（3H-TAN）与大肠杆菌K-12菌株AB 1157和JO 307 rec A uvr A中辐射诱导的DNA瞬变的结合情况。辐照后，将细胞洗涤并重悬于生长培养基中，于37℃放置过夜。在约10%的不确定度范围内，未检测到任何菌株的修复对与DNA结合的TAN产量有影响。在重悬后的时间段内，TAN或TAN片段从辐照的细胞样品中泄漏出来。这种泄漏可能归因于TAN与细胞内辐射诱导的自由基之间的半永久性结合。讨论了TAN与DNA瞬变之间不同相互作用的相关性。
