Kálmán M, Ajtai B M
Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Budapest, Hungary.
Ann Anat. 2000 Sep;182(5):459-63. doi: 10.1016/S0940-9602(00)80056-4.
In the mammalian and avian brains the predominant astroglial elements are astrocytes, and the distribution of GFAP-immunopositivity is rather uneven, some large brain areas being almost devoid of GFAP-immunopositivity. In these areas however, an intense GFAP-immunopositivity appears following injury. In the teleost brain most of the areas are GFAP-immunopositive and ependymoglia predominates. However, a large area, the layer of the sensory neurons in the vagal lobe, is devoid of GFAP. The question arises, whether the lack of GFAP-immunopositivity in the teleost brain is also due to the repression of the GFAP-production, as in birds and mammals, or due to the lack of cells capable of expressing GFAP. To answer this question, stab wounds were made in the vagal lobe of goldfish, as well as in the tectum, in which moderately dense but highly organized GFAP-immunopositive glia has been detected in intact animals. In the layer of the sensory neurons in the vagal lobe no GFAP-immunopositivity appeared even after lesions had been introduced. In the tectum, a rather slight increase of the intensity of the immunostaining was observed in the glial fibers near the lesions but no typical reactive glia similar to that found in mammals or birds, was observed. The results suggest that a lesion does not provoke GFAP-expression in GFAP-immunonegative brain areas in teleosts, in contrast to what is observed in mammals and birds.
在哺乳动物和鸟类的大脑中，主要的星形胶质细胞成分是星形胶质细胞，并且胶质纤维酸性蛋白（GFAP）免疫阳性的分布相当不均匀，一些大脑大区域几乎没有GFAP免疫阳性。然而，在这些区域，损伤后会出现强烈的GFAP免疫阳性。在硬骨鱼的大脑中，大多数区域是GFAP免疫阳性的，室管膜胶质细胞占主导。然而，一个大区域，即迷走叶中的感觉神经元层，没有GFAP。问题出现了，硬骨鱼大脑中缺乏GFAP免疫阳性是像在鸟类和哺乳动物中一样由于GFAP产生的抑制，还是由于缺乏能够表达GFAP的细胞。为了回答这个问题，在金鱼的迷走叶以及顶盖中制造刺伤，在完整动物的顶盖中已经检测到中等密度但高度有组织的GFAP免疫阳性胶质细胞。即使在引入损伤后，迷走叶中的感觉神经元层也没有出现GFAP免疫阳性。在顶盖中，在损伤附近的胶质纤维中观察到免疫染色强度有相当轻微的增加，但没有观察到类似于在哺乳动物或鸟类中发现的典型反应性胶质细胞。结果表明，与在哺乳动物和鸟类中观察到的情况相反，损伤不会在硬骨鱼的GFAP免疫阴性脑区中引发GFAP表达。
