Haugen O A, Skjorten F, Closs O
Acta Pathol Microbiol Scand A. 1975 Nov;83(6):693-703.
Mice of the inbred strains C57BL and C3H were inoculated in the foot-pads with Mycobacterium lepraemurium (MLM) and the inflammatory reaction was studied using light and electron microscopy. In C57BL mice a granulomatous reaction developed 3-4 weeks after inoculation. The inflammatory exudate at this stage showed numerous lymphocytes, monocytes and macrophages. The latter cell type often contained many lysosomes and appeared activated. The bacilli which were all within phagosomes showed extensive electron dense aggregates of the cytoplasm suggesting severe damage. Lymphocytes and macrophages in close contact with each other were often observed. In macrophages which contained damaged bacilli, spherical lipid-like bodies surrounded by granular endoplasmic reticulum were observed. It is suggested that this cell product could be of some significance for the bactericidal function of the macrophage. Contrary to these findings, the cellular infiltrate developing in C3H mice showed no lymphocytes and consisted exclusively of macrophages. These were all heavily loaded with bacilli. The vast majority of bacilli encountered in this strain was morphologically intact and presumably viable. Lipid-like bodies similar to those observed in infected C57BL macrophages were not encountered in C3H mice. It is concluded that unless the infected macrophages become immunologically activited they are unable to cause bacterial damage or to inhibit the growth of MLM.
将近交系C57BL和C3H小鼠的足垫接种鼠麻风杆菌（MLM），并使用光学显微镜和电子显微镜研究炎症反应。在C57BL小鼠中，接种后3 - 4周出现肉芽肿反应。此阶段的炎性渗出物中有大量淋巴细胞、单核细胞和巨噬细胞。后一种细胞类型通常含有许多溶酶体，看起来呈激活状态。所有处于吞噬体中的杆菌都显示出广泛的细胞质电子致密聚集物，提示严重损伤。经常观察到淋巴细胞和巨噬细胞彼此紧密接触。在含有受损杆菌的巨噬细胞中，观察到被颗粒状内质网包围的球形脂质样体。提示这种细胞产物可能对巨噬细胞的杀菌功能具有一定意义。与这些发现相反，C3H小鼠中出现的细胞浸润没有淋巴细胞，仅由巨噬细胞组成。这些巨噬细胞都大量携带杆菌。在该品系中遇到的绝大多数杆菌在形态上是完整的，推测是有活力的。在C3H小鼠中未遇到与感染的C57BL巨噬细胞中观察到的类似脂质样体。得出的结论是，除非受感染的巨噬细胞被免疫激活，否则它们无法造成细菌损伤或抑制MLM的生长。
