Ahluwalia J, Urban L, Capogna M, Bevan S, Nagy I
Department of Anaesthetics, Imperial College Medical School, Chelsea and Westminster Hospital, 369 Fulham Road, SW10 9NH, London, UK.
Neuroscience. 2000;100(4):685-8. doi: 10.1016/s0306-4522(00)00389-4.
Expression of cannabinoid 1 (CB1) and vanilloid 1 (VR1) receptor proteins was studied in adult, cultured rat dorsal root ganglion neurons. Immunostaining of CB1 receptors alone produced labelling in 57+/-2% of the cultured dorsal root ganglion neurons (n=3 cultures). The area of the labelled cells was between 200 and 800 microm(2) with an average of 527+/-68 microm(2). VR1 immunolabelling revealed immunopositivity in 42+/-6% of the total population of dorsal root ganglion neurons. Cells showing VR1-like immunopositivity had an area between 200 and 600 microm(2). The mean area of the VR1-like immunopositive neurons was 376+/-61 microm(2). Double immunostaining with antisera raised against the CB1 and VR1 receptor proteins, showed a high degree of co-expression between CB1 and VR1 receptors. An average of 82+/-3% of the CB1-like immunopositive cells also showed VR1-like immunoreactivity (n=3 cultures) while 98+/-2% of the VR1-like immunolabelled neurons showed CB1 receptor-like immunostaining (n=3 cultures). Our data suggests that nociceptive primary sensory neurons co-express CB1 and VR1 receptors to a very high degree. We propose that this may provide an anatomical basis for a powerful combination of VR1 mediated excitation and CB1-mediated inhibition of nociceptive responses at central and peripheral terminals of nociceptive primary afferents.
在成年大鼠背根神经节神经元培养物中研究了大麻素1（CB1）和香草酸受体1（VR1）受体蛋白的表达。单独对CB1受体进行免疫染色，在57±2%的培养背根神经节神经元中产生标记（n = 3个培养物）。标记细胞的面积在200至800平方微米之间，平均为527±68平方微米。VR1免疫标记显示在背根神经节神经元总数的42±6%中呈免疫阳性。显示VR1样免疫阳性的细胞面积在200至600平方微米之间。VR1样免疫阳性神经元的平均面积为376±61平方微米。用针对CB1和VR1受体蛋白的抗血清进行双重免疫染色，显示CB1和VR1受体之间存在高度共表达。平均82±3%的CB1样免疫阳性细胞也显示VR1样免疫反应性（n = 3个培养物），而98±2%的VR1样免疫标记神经元显示CB1受体样免疫染色（n = 3个培养物）。我们的数据表明，伤害性初级感觉神经元高度共表达CB1和VR1受体。我们提出，这可能为VR1介导的兴奋与CB1介导的对伤害性初级传入神经中枢和外周终末伤害性反应抑制的强大组合提供解剖学基础。
