Maas M R, Norgren R B
Department of Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha 68198-6395, USA.
Tissue Cell. 2000 Jun;32(3):216-22. doi: 10.1054/tice.2000.0105.
The olfactory placode gives rise to both olfactory receptor neurons, which remain as a component of the peripheral nervous system, and to luteinizing hormone-releasing hormone (LHRH) neurons, which migrate to the central nervous system. In this study, we used chick olfactory placode explants to ask several questions regarding LHRH neuronal differentiation. We found that explants of ectoderm from the fronto-nasal region of embryos as early as Hamilton & Hamburger (HH) stage 12 gave rise to LHRH neurons, that explants from all regions of the olfactory placode were able to generate LHRH neurons, that both brain conditioned medium and disruption of the olfactory placode increase the number of LHRH neurons observed in explants, and that the combination of these two manipulations results in the production of more LHRH neurons than either treatment alone. We conclude that LHRH neurons originate in the olfactory epithelium and that some of the same factors which influence olfactory receptor neuron development also affect LHRH neuronal development.
嗅基板可产生嗅觉受体神经元（其仍作为外周神经系统的一个组成部分）以及促黄体生成激素释放激素（LHRH）神经元（其迁移至中枢神经系统）。在本研究中，我们使用鸡胚嗅基板外植体来探讨几个关于LHRH神经元分化的问题。我们发现，早在汉密尔顿和汉伯格（HH）第12阶段的胚胎额鼻区域的外胚层外植体可产生LHRH神经元，嗅基板所有区域的外植体都能够产生LHRH神经元，脑条件培养基和嗅基板的破坏均可增加在外植体中观察到的LHRH神经元数量，并且这两种操作的组合所产生的LHRH神经元比单独任何一种处理都要多。我们得出结论，LHRH神经元起源于嗅觉上皮，并且一些影响嗅觉受体神经元发育的相同因素也会影响LHRH神经元的发育。
