Thomas A C, Campbell J H
Centre for Research in Vascular Biology, Department of Anatomical Sciences, The University of Queensland, Brisbane, Australia.
Tissue Cell. 2000 Jun;32(3):249-56. doi: 10.1054/tice.2000.0113.
The aim of this study was to determine whether similar populations of smooth muscle cells, in relation to contractile and cytoskeletal proteins, are present in normal and diseased human coronary arteries and normal and injured rat and rabbit arteries. Rat aortae and rabbit carotid arteries were de-endothelialised and the resulting neointimal thickening examined at set time points 2-24 weeks later. Immunohistochemistry revealed that arteries had three distinct populations of cells in respect to alpha-smooth muscle actin, smooth muscle myosin heavy chain and vimentin (staining intensities '-', '+' or '++' for each protein), but only two populations in respect to desmin ('-' and '+'). The different populations of cells were found in the neointima at all times after injury, in human atherosclerotic plaque and in the media of diseased, injured and uninjured vessels, although in different proportions. It was concluded that arteries of the human, rat and rabbit have cells with a wide spectrum of contractile and cytoskeletal proteins. Expression of the different proteins did not reflect the state of the artery after injury or during the disease process, and was not associated with the expansion of a subset of cells within the artery wall.
本研究的目的是确定在正常和病变的人类冠状动脉以及正常和受损的大鼠及兔动脉中，是否存在与收缩蛋白和细胞骨架蛋白相关的相似平滑肌细胞群体。对大鼠主动脉和兔颈动脉进行去内皮处理，并在2至24周后的设定时间点检查由此产生的新生内膜增厚情况。免疫组织化学显示，就α-平滑肌肌动蛋白、平滑肌肌球蛋白重链和波形蛋白而言（每种蛋白的染色强度为“-”、“+”或“++”），动脉中有三种不同的细胞群体，但就结蛋白而言（“-”和“+”）只有两种细胞群体。损伤后的所有时间点，在人类动脉粥样硬化斑块以及病变、受损和未受损血管的中膜中，均发现了不同的细胞群体，尽管比例不同。得出的结论是，人类、大鼠和兔的动脉具有具有广泛收缩蛋白和细胞骨架蛋白的细胞。不同蛋白的表达并未反映损伤后或疾病过程中动脉的状态，也与动脉壁内细胞亚群的扩张无关。
