Hasegawa H, Suzuki K, Suzuki K, Nakaji S, Sugawara K
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori, 036-8562, Japan.
Luminescence. 2000 Sep-Oct;15(5):321-7. doi: 10.1002/1522-7243(200009/10)15:5<321::AID-BIO605>3.0.CO;2-O.
To investigate the effects of zinc on non-specific immune functions, we used the chemiluminescence method to examine the capacity of human neutrophils to produce reactive oxygen species and accompanying serum opsonic activity. When neutrophils were stimulated with both opsonized zymosan and phorbol myristate acetate in the presence of 1-10(-3) mmol/L zinc lucigenin-dependent CL responses were stable or declined, whereas luminol-dependent CL responses were significantly enhanced. The results suggest that zinc activates protein kinase C and promotes MPO degranulation and ROS metabolism, especially in hypochlorous acid production, which have the direct action of causing microbial death. Further, the lucigen-dependent CL response stimulated with OZ was strongly enhanced by anti-MPO antibodies, whereas the enhancement was less in the presence of zinc, suggesting that zinc may suppress the receptor-mediated signal transduction process. Both responses were inhibited at 10 mmol/L. Serum opsonic activity was enhanced by zinc at 10(-4) and 10(-3) mmol/L but reduced at 10 mmol/L. These data indicate that addition of zinc around and above normal physiological concentrations facilitates neutrophil functional activity and serum opsonic activity, whereas these are inhibited by a lack of zinc or an excessive amount, suggesting that zinc is essential for optimal functioning of non-specific immunity.
为研究锌对非特异性免疫功能的影响，我们采用化学发光法检测人中性粒细胞产生活性氧的能力以及伴随的血清调理活性。当中性粒细胞在1-10（-3）mmol/L锌存在的情况下，用调理酵母聚糖和佛波酯肉豆蔻酸酯刺激时，基于光泽精的化学发光反应稳定或下降，而基于鲁米诺的化学发光反应显著增强。结果表明，锌激活蛋白激酶C并促进髓过氧化物酶脱颗粒和活性氧代谢，特别是在次氯酸生成方面，这具有导致微生物死亡的直接作用。此外，用抗髓过氧化物酶抗体可强烈增强由酵母聚糖刺激的基于光泽精的化学发光反应，而在锌存在的情况下增强作用较小，这表明锌可能抑制受体介导的信号转导过程。两种反应在10 mmol/L时均受到抑制。锌在10（-4）和10（-3）mmol/L时可增强血清调理活性，但在10 mmol/L时则降低。这些数据表明，在正常生理浓度及以上添加锌可促进中性粒细胞功能活性和血清调理活性，而锌缺乏或过量则会抑制这些活性，这表明锌对于非特异性免疫的最佳功能至关重要。
