Gao W, Jiang N, Zhu G
First Hospital of Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1998 Nov;78(11):821-3.
To identify hyperhomocysteinemia (HHe) as a new and independent risk factor for coronary heart disease (CHD).
The association of coronary heart disease (CHD) and lipid, homocysteine (HCY) and the factors related to its metabolisms were examined. The mutation of the 677C-->T transition of MTHFR was determined by PCR-based assay. Whole blood and plasma folate (FA) and plasma vitamin B12(B12), as cofactors of those enzymes, were determined by radioimmunologic assay. Plasma HCY was determined by HPLC.
Patients with CHD confirmed by coronary angiography had increased plasma HCY concentrations (17.1 +/- 3.6 mumol/L, 7.6 +/- 1.2 mumol/L). In patients with MI, HPT and family history (FH) of CHD, plasma HCY increased significantly. Plasma HCY concentrations had significant non-linear inverse relation with plasma FA and B12 concentrations. Homozygous mutants showed higher plasma HCY concentrations. Patients with CHD had increased serum CHOL and VLDL levels, but plasma HCY concentrations were not correlated with serum lipid levels.
Hyperhomocysteinemia is an independent risk factor for coronary heart disease.
确定高同型半胱氨酸血症（HHe）为冠心病（CHD）的一种新的独立危险因素。
研究冠心病（CHD）与血脂、同型半胱氨酸（HCY）及其代谢相关因素之间的关联。采用基于聚合酶链反应（PCR）的检测方法确定亚甲基四氢叶酸还原酶（MTHFR）677C→T突变。作为这些酶的辅助因子，采用放射免疫分析法测定全血和血浆叶酸（FA）以及血浆维生素B12（B12）。采用高效液相色谱法（HPLC）测定血浆HCY。
经冠状动脉造影确诊的冠心病患者血浆HCY浓度升高（17.1±3.6μmol/L，7.6±1.2μmol/L）。在心肌梗死（MI）、冠心病家族史（FH）患者中，血浆HCY显著升高。血浆HCY浓度与血浆FA和B12浓度呈显著非线性负相关。纯合突变体血浆HCY浓度较高。冠心病患者血清胆固醇（CHOL）和极低密度脂蛋白（VLDL）水平升高，但血浆HCY浓度与血脂水平无关。
高同型半胱氨酸血症是冠心病的独立危险因素。
