Wang A, Lu C, Qiao J
Department of Gynecology & Obstetrics, Third Hospital, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1998 Nov;78(11):830-2.
To investigate steroids production of granulosa cells obtained from normal ovaries and polycystic ovaries from polycystic ovary syndrome (PCOS) patients under FSH, LH and insulin stimulation.
Granulosa cells of 33 folicles from 18 pairs of normal ovaries and about 100 folicles from 8 pairs of PCOS were cultured. Estrodial (E2) and progesterone (P) accumulation in the medium was determined under different incubated conditions by RIA.
Granulosa cells from PCOS produced more E2(P < 0.05-0.01) under basal, FSH and insulin stimulation and more P (P < 0.05-0.01) under FSH, LH and insulin stimulation than controls. Insulin markedly augmented FSH and LH-stimulated steroid accumulation by two groups of granulosa cells. Granulosa cells from PCOS with peripheral insulin resistance also responded to insulin.
Enzymes for steroids synthesis in granulosa cells from PCOS may be activated in vivo, and hyperinsulinisma and elevated LH levels in PCOS may be contributable.
研究在促卵泡生成素（FSH）、促黄体生成素（LH）和胰岛素刺激下，从正常卵巢及多囊卵巢综合征（PCOS）患者的多囊卵巢中获取的颗粒细胞的类固醇生成情况。
培养来自18对正常卵巢的33个卵泡的颗粒细胞以及来自8对PCOS患者的约100个卵泡的颗粒细胞。通过放射免疫分析法（RIA）测定在不同培养条件下培养基中雌二醇（E2）和孕酮（P）的积累量。
在基础状态、FSH和胰岛素刺激下，PCOS患者的颗粒细胞产生的E2更多（P<0.05 - 0.01）；在FSH、LH和胰岛素刺激下，其产生的P更多（P<0.05 - 0.01）。胰岛素显著增强了两组颗粒细胞在FSH和LH刺激下的类固醇积累。PCOS患者外周胰岛素抵抗的颗粒细胞也对胰岛素有反应。
PCOS患者颗粒细胞中类固醇合成酶可能在体内被激活，PCOS患者的高胰岛素血症和LH水平升高可能是其原因。
