He W, Wu H L
College of Life Sciences, Peking University, Beijing, China.
Shi Yan Sheng Wu Xue Bao. 1997 Dec;30(4):363-73.
While producing transgenic mice by ES cell route, it is important to know whether ES cells used have a strong ability to produce chimeras or not. In this report, two methods were used for studying the chimeric ability of two ES cell lines (HDC and MESPU-13). One method was the blastocyst microinjection, that is, chimeras were produced by injecting 15 ES cells into the cavity of C57BL/6J blastocysts. Another was GPI electrophoresis for examinizing the chimerism of ES cells in internal tisssues and organs of chimeras. The results showed that the ability of producing chimeras of MESPU-13 cells was stronger than that of HDC cells. The reason was discussed in detail.
通过胚胎干细胞途径制备转基因小鼠时，了解所使用的胚胎干细胞是否具有强大的产生嵌合体的能力非常重要。在本报告中，使用了两种方法来研究两种胚胎干细胞系（HDC和MESPU - 13）的嵌合能力。一种方法是囊胚显微注射，即通过将15个胚胎干细胞注射到C57BL / 6J囊胚的腔内来产生嵌合体。另一种是糖磷脂酰肌醇（GPI）电泳，用于检测嵌合体内部组织和器官中胚胎干细胞的嵌合情况。结果表明，MESPU - 13细胞产生嵌合体的能力强于HDC细胞。并对原因进行了详细讨论。
