[金黄色葡萄球菌乙酰辅酶A合成酶的研究]
[Study of acetyl-CoA-synthetase from staphylococcus aureus].
作者信息
Ignatov V V, Stupnikova S K
出版信息
Biokhimiia. 1975 Mar-Apr;40(2):276-80.
PMID:1104
Abstract
Acetyl-CoA-synthetase was isolated from cells of St. aureus 209-P. The method of isolation and partial purification of the enzyme is worked out. Km values of the enzyme for acetate, CoA and ATP are calculated. p-Chloromercuribenzoate and monoiodoacetate were shown to inhibit the enzyme activity. The enzyme activity is estimated depending on the age of the cell culture and on the presence of acetate in the culture medium.
摘要
从金黄色葡萄球菌209-P细胞中分离出乙酰辅酶A合成酶。制定了该酶的分离和部分纯化方法。计算了该酶对乙酸盐、辅酶A和三磷酸腺苷的米氏常数。已证明对氯汞苯甲酸和一碘乙酸可抑制该酶的活性。根据细胞培养物的年龄和培养基中乙酸盐的存在情况来估计酶的活性。
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