Gerhardt H, Walz M J, Faigle M, Northoff H, Wolburg H, Neumeister B
Pathologisches Institut der Universität Tübingen, Tübingen, Germany.
FEMS Microbiol Lett. 2000 Nov 1;192(1):145-52. doi: 10.1111/j.1574-6968.2000.tb09373.x.
In this report, we investigate the intracellular fate of selected members of the genus Legionella within the monocytic cell line Mono Mac 6 cells. By means of electron microscopy and immunocytochemistry, we could show that Legionella pneumophila as well as Legionella longbeachae are able to induce ribosome-studded phagosomes which associate with the rough endoplasmic reticulum (RER), whereas Legionella micdadei remains to be located within smooth phagosomes but also shows signs of RER association. In addition, we could demonstrate a remarkable correlation between the phagosome type and the morphological phenotype of intracellular bacteria: within ribosome-studded phagosomes, bacteria generally lacked the outer coat of low electron density whereas bacteria within the smooth phagosomes still possessed this outer coat. The virulence factors responsible for inhibition of phagosome maturation and their distribution within the genus Legionella as well as the biological significance of the morphological difference of bacteria within smooth and ER-associated phagosomes remain to be investigated.
在本报告中，我们研究了军团菌属选定成员在单核细胞系Mono Mac 6细胞内的细胞内命运。通过电子显微镜和免疫细胞化学方法，我们能够证明嗜肺军团菌以及长滩军团菌能够诱导与糙面内质网（RER）相关的核糖体附着吞噬体，而米克戴德军团菌仍位于光滑吞噬体内，但也显示出与RER相关的迹象。此外，我们能够证明吞噬体类型与细胞内细菌的形态表型之间存在显著相关性：在核糖体附着吞噬体内，细菌通常缺乏低电子密度的外膜，而在光滑吞噬体内的细菌仍具有这种外膜。负责抑制吞噬体成熟的毒力因子及其在军团菌属内的分布，以及光滑和内质网相关吞噬体内细菌形态差异的生物学意义仍有待研究。
