Chromogranin A-immunoreactive cells in the olfactory system of anuran amphibians.

Chromogranin A (CgA) is a member of the granin family of acidic proteins that are present in the secretory granules of many endocrine and neuroendocrine cells. The specific function(s) of these proteins is not known, but they seem to be the precursors of biologically active peptides, and they may act as helper proteins in the sorting and packaging of peptide hormones and neuropeptides. Using indirect immunohistochemistry, we have found CgA immunoreactivity in the primary olfactory epithelia, the vomeronasal epithelia, the olfactory nerves, and the olfactory bulbs of tadpoles of the American toad, Bufo americanus, and the green frog, Rana clamitans. CgA immunoreactivity was present in the early stages of larval development in toads but was not detected in toad tadpoles after the hindlimb buds formed or in toadlets or adults. In green frog tadpoles, CgA-immunoreactive cells were found in pre- and prometamorphic stages but not in late climax. CgA immunoreactivity was also absent in froglets, but it was detected in the vomeronasal epithelium but not the olfactory epithelium of adult green frogs.