Boffi A, Rizzi M, Monacelli F, Ascenzi P
CNR, Centro di Biologia Molecolare and Dipartimento di Scienze Biochimiche 'Alessandro Rossi Fanelli', Università di Roma 'La Sapienza', Rome, Italy.
Biochim Biophys Acta. 2000 Oct 18;1523(2-3):206-8. doi: 10.1016/s0304-4165(00)00123-9.
A new, simple and fast spectrophotometric method for the determination of the H(2)S concentration is reported. This method, based on the 1:1 reaction between H(2)S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H(2)S dissolved in a given solution even at concentrations as low as 1 x 10(-6) M. Note that L. pectinata HbI is considered the physiological receptor of H(2)S.
报道了一种用于测定硫化氢(H₂S)浓度的新型、简单且快速的分光光度法。该方法基于双壳贝类软体动物栉孔扇贝血红蛋白I(HbI)的铁衍生物与H₂S之间的1:1反应,即使在给定溶液中溶解的H₂S浓度低至1×10⁻⁶ M时,也能对其进行定量测定。请注意,栉孔扇贝HbI被认为是H₂S的生理受体。