钙离子对大鼠肝脏线粒体内膜氧化磷酸化的调节作用。
Regulation of oxidative phosphorylation in the inner membrane of rat liver mitochondria by calcium ions.
作者信息
Evtodienko Y V, Azarashvili T S, Teplova V V, Odinokova I V, Saris N
机构信息
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia.
出版信息
Biochemistry (Mosc). 2000 Sep;65(9):1023-6.
The effect of accumulation of Ca2+ at physiological concentrations (10(-8)-10(-6) M) on the rates of ATP synthesis and hydrolysis in rat liver mitochondria was studied. An addition of 5 x 10(-7) M Ca2+ resulted in the maximal rates of synthesis and hydrolysis of ATP. Decrease in the concentration of Ca2+ to 10-8 M or its increase to 5 x 10(-6) M inhibited oxidative phosphorylation and ATP hydrolysis. It was found that the rate of oxidative phosphorylation correlated with the phosphorylation level of a 3.5-kD peptide in the mitochondrial inner membrane on varying the Ca2+ concentration. The possible regulation of oxidative phosphorylation in mitochondria by Ca2+ is discussed.
研究了生理浓度（10⁻⁸ - 10⁻⁶ M）的Ca²⁺积累对大鼠肝脏线粒体中ATP合成和水解速率的影响。添加5×10⁻⁷ M的Ca²⁺可导致ATP合成和水解的最大速率。将Ca²⁺浓度降低至10⁻⁸ M或升高至5×10⁻⁶ M会抑制氧化磷酸化和ATP水解。研究发现，在改变Ca²⁺浓度时，氧化磷酸化速率与线粒体内膜中一种3.5-kD肽的磷酸化水平相关。文中讨论了Ca²⁺对线粒体氧化磷酸化可能的调节作用。
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