Ignatov O V, Grechkina E V, Muratova A Iu, Turkovskaia O V, Ignatov V V
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
Prikl Biokhim Mikrobiol. 2000 Sep-Oct;36(5):555-8.
The respiratory activity of Acinetobacter calcoaceticus TM-31 with resect to alkane hydrocarbons was studied. The dynamics of oxygen consumption by the cells while assimilating n-hexadecane was assayed by a modified technique using an oxygen electrode. The dependence of cell respiratory activity on the amount of n-hexadecane within the concentration range of 0.03-0.66% was determined. It was demonstrated that the cells also displayed respiratory activity towards other medium-chain n-alkanes: hexane, octane, decane, tridecane, and heptadecane. Thus, we demonstrated the possibility of determining alkanes by measuring the respiratory activities of microorganisms.
研究了醋酸钙不动杆菌TM-31对烷烃的呼吸活性。采用改良的氧电极技术测定了细胞在同化正十六烷时的耗氧动力学。确定了在0.03-0.66%浓度范围内细胞呼吸活性对正十六烷量的依赖性。结果表明,细胞对其他中链正构烷烃,如己烷、辛烷、癸烷、十三烷和十七烷也表现出呼吸活性。因此,我们证明了通过测量微生物的呼吸活性来测定烷烃的可能性。