Hilgert M, Buchholzer M, Jeltsch H, Kelche C, Cassel J C, Klein J
Department of Pharmacology, University of Mainz, Germany.
Neuroreport. 2000 Sep 28;11(14):3063-5. doi: 10.1097/00001756-200009280-00006.
Adult female rats sustained aspirative fimbria-fornix lesions and, 2 weeks later, received intrahippocampal grafts of fetal septal or mixed septal-raphe cell suspensions. Twenty-four months later, the extracellular concentration of hippocampal acetylcholine (ACh) was determined by microdialysis. Basal ACh levels (5-65 fmol/5 microl sham-operated rats) were strongly reduced after lesioning (3-7 fmol/5 microl). In septally transplanted and septal-raphe co-transplanted rats, hippocampal ACh concentrations were restored to near-normal levels (15-25 fmol/5 microl), indicating long-term functional survival of hippocampal transplants. After administration of citalopram (100 microM by infusion) and fenfluramine (20 mg/kg i.p.), the hippocampal ACh efflux was increased by 2- to 3-fold in all groups of rats. The relative increase of ACh was highest in co-transplanted rats, an effect which was possibly due to functional interactions between grafted raphe and septal neurons.
成年雌性大鼠遭受了抽吸性穹窿海马伞损伤，两周后接受了海马内胎儿隔区或隔区-中缝混合细胞悬液移植。24个月后，通过微透析测定海马乙酰胆碱（ACh）的细胞外浓度。基础ACh水平（假手术大鼠为5 - 65 fmol/5微升）在损伤后大幅降低（3 - 7 fmol/5微升）。在接受隔区移植和隔区-中缝联合移植的大鼠中，海马ACh浓度恢复到接近正常水平（15 - 25 fmol/5微升），表明海马移植具有长期功能存活。给予西酞普兰（通过灌注100微摩尔）和芬氟拉明（腹腔注射20毫克/千克）后，所有组大鼠的海马ACh流出量增加了2至3倍。联合移植大鼠中ACh的相对增加最高，这一效应可能归因于移植的中缝和隔区神经元之间的功能相互作用。
