Dassesse D, Ledent C, Meunier J C, Parmentier M, Schiffmann S N
Department of Neuroscience, School of Medicine, Université Libre de Bruxelles, Brussels, Belgium.
Neuroreport. 2000 Sep 28;11(14):3243-6. doi: 10.1097/00001756-200009280-00038.
Most effects of nociceptin are related to blockade of stress and anxiolytic-like effects. This neuropeptide is highly expressed in septal nuclei, which are involved in response to stressful situations. Dopamine and adenosine may have modulatory effects on stress behaviour by acting on septal neurons. We therefore analysed the regulation of septal nociceptin expression using quantitative in situ hybridization following manipulations of adenosine and dopamine neurotransmission. No difference was observed between wild-type and A2A receptor-deficient mice. In both genotypes, chronic treatments with caffeine, an equipotent A1 and A2A adenosine receptor antagonist, did not significantly modify nociceptin expression. 6-Hydroxydopamine-induced dopamine depletion was also without effect. These results demonstrate that dopamine and adenosine are not involved in the regulation of septal nociceptin expression in spite of the involvement of these three neurotransmitters in stress and anxiety behaviours.
孤啡肽的大多数作用与应激阻断和抗焦虑样作用有关。这种神经肽在隔核中高度表达，隔核参与对应激情况的反应。多巴胺和腺苷可能通过作用于隔核神经元对应激行为产生调节作用。因此，我们在对腺苷和多巴胺神经传递进行操作后，使用定量原位杂交分析了隔核孤啡肽表达的调控。在野生型和A2A受体缺陷型小鼠之间未观察到差异。在两种基因型中，使用咖啡因（一种等效的A1和A2A腺苷受体拮抗剂）进行慢性治疗，并未显著改变孤啡肽的表达。6-羟基多巴胺诱导的多巴胺耗竭也没有效果。这些结果表明，尽管这三种神经递质参与应激和焦虑行为，但多巴胺和腺苷并不参与隔核孤啡肽表达的调控。
