缺血耐受会影响谷氨酸受体2（GluR2）信使核糖核酸（mRNA）的腺苷化状态。

Ischemic tolerance affects the adenylation state of GluR2 mRNA.

作者信息

Alsbo C W, Wrang M L, Nielsen M, Diemer N H

机构信息

Laboratory of Neuropathology, Institute of Molecular Pathology, University of Copenhagen, Denmark.

出版信息

Neuroreport. 2000 Sep 28;11(14):3279-82. doi: 10.1097/00001756-200009280-00046.

DOI:10.1097/00001756-200009280-00046

PMID:11043564

Abstract

To analyse GluR2 regulations in the rat hippocampal CA1 region following global and tolerance-inducing ischemia in situ hybridization (ISH) and quantitative PCR (Q-PCR) was applied. In addition, cDNA was synthesised from two different primer combinations in order to elucidate possible differences in the adenylation state of GluR2 mRNA. Following global ischemia, ISH and Q-PCR both showed reductions to half of control levels of GluR2 mRNA in consent with previously published results. Following tolerance induction, ISH showed no changes, whereas PCR analysis showed up-regulation to 228% of control value for the general cDNA synthesis, and no change for the specific cDNA synthesis. This indicates that tolerance-inducing ischemia does not increase the amount of GluR2 mRNA; instead polyadenylation of the existing GluR2 mRNA pool takes place.

摘要

为分析在大鼠海马CA1区全脑缺血及诱导耐受缺血后GluR2的调控情况，采用了原位杂交（ISH）和定量聚合酶链反应（Q-PCR）技术。此外，从两种不同的引物组合合成了互补DNA（cDNA），以阐明GluR2信使核糖核酸（mRNA）腺苷酸化状态可能存在的差异。全脑缺血后，ISH和Q-PCR均显示GluR2 mRNA水平降至对照水平的一半，这与先前发表的结果一致。诱导耐受后，ISH显示无变化，而聚合酶链反应（PCR）分析表明，对于一般的cDNA合成，上调至对照值的228%，而特异性cDNA合成无变化。这表明诱导耐受的缺血并未增加GluR2 mRNA的量；相反，现有GluR2 mRNA库发生了多聚腺苷酸化。