携带野生型p53基因的逆转录病毒载体及其对人胶质瘤细胞的作用。
Retrovirus vector containing wild type p53 gene and its effect on human glioma cells.
作者信息
Hlavatý J, Tyukosova S, Bies J, Hlubinova K, Altaner C
机构信息
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
出版信息
Neoplasma. 2000;47(4):204-11.
A retroviral vector containing wild-type p53 tumor suppressor gene (wt-p53) under the control of viral LTR sequences was constructed and transfected into packaging cell line GP+envAm12. Virus producing single cell clone GP+envAm12/ p53clC8 (8 x 10(5) cfu/ml, determined on NIH 3T3 cells) was isolated and used to transfer wt-p53 gene into human glioma cell lines in vitro. Decreased viability in p53-infected cells as compared to uninfected or empty virus infected cells was observed.
构建了一种逆转录病毒载体，其在病毒长末端重复序列（LTR）序列的控制下含有野生型p53肿瘤抑制基因（wt-p53），并将其转染到包装细胞系GP + envAm12中。分离出产生病毒的单细胞克隆GP + envAm12 / p53clC8（在NIH 3T3细胞上测定为8×10⁵ cfu/ml），并用于在体外将wt-p53基因转移到人胶质瘤细胞系中。与未感染或空病毒感染的细胞相比，观察到p53感染细胞的活力降低。
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