[Construction of the wt-p53 gene recombinant retroviral vector. Inhibiting the growth of laryngocarcinoma cell line].

We have constructed wt-p53 gene recombinant retroviral vector. The p53 cDNA with positive regulative sequence was subcloned into the Xho I site between the two LTRs of the N2A retroviral vector which contained the cytomegalovirus promotor/enhancer for expression and a neomycin resistance gene allowing G418 selection in reverse orientation and established the PA317 packaging cell line producing virus. The recipient cell line of Hep2 (laryngocarcinoma) containing the abnormal p53 gene was transfected in vitro with fresh retroviral stock produced. The result showed that the constructed wt-p53 gene recombinant retroviral vector was able to suppress growth of laryngocarcinoma cell in vitro. These observations indicate that recombinant retrovirus expressing wild-type p53 may be the useful vectors for gene therapy of human laryngocarcinoma.