在小鼠和绵羊中表达绵羊带绦虫45W、18k和16k宿主保护性抗原的DNA疫苗的比较。

A comparison of DNA vaccines expressing the 45W, 18k and 16k host-protective antigens of Taenia ovis in mice and sheep.

作者信息

Drew D R, Lightowlers M W, Strugnell R A

机构信息

Department of Microbiology and Immunology, The University of Melbourne, Parkville, Vic.,

出版信息

Vet Immunol Immunopathol. 2000 Oct 31;76(3-4):171-81. doi: 10.1016/s0165-2427(00)00206-3.

DOI:10.1016/s0165-2427(00)00206-3

PMID:11044551

Abstract

The immunogenicity of DNA vaccines encoding three different Taenia ovis host-protective antigens was compared in mice and sheep. DNA vaccines encoding the 45W, 18k and 16k antigens of T. ovis were constructed. The ability of DNA vaccines encoding the 45W and 18k genes to express antigen was confirmed by Western blotting of transfected Cos-7 cells. BALB/c mice were vaccinated intramuscularly with 45W, 18k or 16k DNA vaccines and the humoral immune response analysed by ELISA. DNA vaccines expressing 45W, 18k or 16k antigen were immunogenic in mice and generated significant titres of antigen-specific antibody. Intramuscular vaccination of outbred sheep with the T. ovis DNA vaccines generated significantly lower titres of 45W-specific antibody and failed to generate 18k or 16k-specific antibody. The findings of this study show that each of the three T. ovis host-protective antigens are amenable to delivery via DNA vaccines, and that the parameters governing the efficacy of DNA vaccines in sheep require further investigation.

摘要

在小鼠和绵羊中比较了编码三种不同绵羊绦虫宿主保护性抗原的DNA疫苗的免疫原性。构建了编码绵羊绦虫45W、18k和16k抗原的DNA疫苗。通过对转染的Cos-7细胞进行蛋白质免疫印迹分析，证实了编码45W和18k基因的DNA疫苗表达抗原的能力。将BALB/c小鼠肌肉注射接种45W、18k或16k DNA疫苗，并通过酶联免疫吸附测定法分析体液免疫反应。表达45W、18k或16k抗原的DNA疫苗在小鼠中具有免疫原性，并产生了显著滴度的抗原特异性抗体。用绵羊绦虫DNA疫苗对远交群绵羊进行肌肉注射接种，产生的45W特异性抗体滴度显著较低，且未能产生18k或16k特异性抗体。本研究结果表明，三种绵羊绦虫宿主保护性抗原中的每一种都适合通过DNA疫苗递送，并且控制DNA疫苗在绵羊中效力的参数需要进一步研究。