兔实验性肾盂肾炎中的局部免疫反应。II. 感染生物体脂多糖对淋巴细胞的刺激
Local immune response in experimental pyelonephritis in the rabbit. II. Lymphocyte stimulation by lipopolysaccharide of infecting organism.
作者信息
Smith J W
出版信息
Immunology. 1975 Dec;29(6):1077-85.
The response of circulating and kidney lymphocytes from rabbits with experimental haematogenous pyelonephritis to somatic (O) antigen (lipopolysaccharide) from the infecting organism was evaluated. Lymphocytes were cultured for 3 days in the presence and absence of lipopolysaccharide from the infecting organism, Escherichia coli O75 and a heterologous organism. Circulating lymphocytes showed a significant response to the homologous lipopolysaccharide by day 5 of infection, and kidney lymphocytes responded by day 19 of infection but failed to respond after day 33. The lipid moiety of somatic antigen was required for the stimulation of lymphocytes since antigen preparations in which lipid was low or absent did not activate lymphocytes. B lymphocytes from kidney were stimulated by this antigen whereas both T and B circulating lymphocytes responded. Thus, pyelonephritis in rabbits is associated with the activation of a subpopulation of circulating cells which have specific receptors for the homologous antigen. B lymphocytes that are specifically activated by the homologous or O antigen then localize to the infected site.
对患有实验性血源性肾盂肾炎的兔子的循环淋巴细胞和肾淋巴细胞对感染生物体的菌体（O）抗原（脂多糖）的反应进行了评估。淋巴细胞在有和没有来自感染生物体大肠杆菌O75及一种异源生物体的脂多糖存在的情况下培养3天。循环淋巴细胞在感染第5天时对同源脂多糖有显著反应，肾淋巴细胞在感染第19天时做出反应，但在第33天后不再有反应。由于脂质含量低或没有脂质的抗原制剂不能激活淋巴细胞，所以菌体抗原的脂质部分是刺激淋巴细胞所必需的。这种抗原能刺激来自肾脏的B淋巴细胞，而循环中的T和B淋巴细胞均有反应。因此，兔子的肾盂肾炎与循环细胞亚群的激活有关，这些循环细胞对同源抗原有特异性受体。被同源或O抗原特异性激活的B淋巴细胞随后定位于感染部位。
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