兔实验性肾盂肾炎中的局部免疫反应。III. 淋巴细胞对大肠杆菌O抗原和K抗原的反应性
Local immunie response in experimental pyelonephritis in the rabbit. III. Lymphocyte responsivieness to O and K antigens of Escherichia coli.
作者信息
Smith J W, Kaijser B
出版信息
Immunology. 1976 Aug;31(2):233-7.
The response of circulating and infected kidney lymphocytes to the O (lipopolysaccharide) and K (polysaccharide) antigens of an Escherichia coli O6 K 13 H1 strain was determined. Both circulating and kidney lymphocytes showed significant incorporation of [3H-methyl]thymidine into DNA when incubated with the O antigen, whereas neither responded to the K antigen. The lipid moiety of the lipopolysaccharide was required for lymphocyte responsiveness. Upon sequential incubation of O antigen and fluoresceinated homologous antiserum, 24-30 per cent of kidney lymphocytes were shown to have surface receptors for O antigen, whereas none had surface receptors for K antigen. Although the K antigen is an important determinant of invasiveness of the upper urinary tract, it fails to elicit a cellular immune response or attach to lymphocytes from the infected kidney in experimental pyelonephritis.
测定了循环及感染肾脏淋巴细胞对大肠杆菌O6 K13 H1菌株的O（脂多糖）和K（多糖）抗原的反应。当与O抗原一起孵育时，循环及肾脏淋巴细胞均显示出显著地将[3H-甲基]胸苷掺入DNA，而两者对K抗原均无反应。脂多糖的脂质部分是淋巴细胞反应性所必需的。对O抗原和荧光素化的同源抗血清进行连续孵育后，显示24% - 30%的肾脏淋巴细胞具有O抗原的表面受体，而没有一个具有K抗原的表面受体。虽然K抗原是上尿路侵袭性的一个重要决定因素，但在实验性肾盂肾炎中，它未能引发细胞免疫反应或附着于感染肾脏的淋巴细胞。
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