Benhammou K, Lee M, Strook M, Sullivan B, Logel J, Raschen K, Gotti C, Leonard S
Department of Psychiatry, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262, USA.
Neuropharmacology. 2000 Oct;39(13):2818-29. doi: 10.1016/s0028-3908(00)00153-2.
The principal sites for biological action of tobacco products are thought to be the nicotinic acetylcholine receptors (nAChR). Nicotinic receptor subunit genes, therefore, represent an important gene family for study in nicotine addiction. They are localized in both brain and in the periphery. In brain these receptors appear to function as modulators of synaptic transmission; the function of peripheral receptors is not known. Nicotinic receptor levels in human brain are regulated by smoking in a dose-dependent manner. In peripheral blood, nicotinic receptors are present on both lymphocytes and polymorphonuclear cells (PMN). We have compared [(3)H]nicotine binding in PMN isolated from smokers and non-smokers. [(3)H]nicotine binding was increased in smokers and was correlated, as in brain, with tobacco use. Expression of both mRNA and protein in lymphocytes and PMN, for a subset of nicotinic receptor subunits, suggests that these cell types contain both alpha4beta2 and alpha3beta4 receptors.
烟草制品的主要生物作用位点被认为是烟碱型乙酰胆碱受体（nAChR）。因此，烟碱型受体亚基基因是研究尼古丁成瘾的一个重要基因家族。它们定位于大脑和外周。在大脑中，这些受体似乎作为突触传递的调节剂发挥作用；外周受体的功能尚不清楚。人脑中烟碱型受体水平受吸烟的剂量依赖性调节。在外周血中，淋巴细胞和多形核细胞（PMN）上均存在烟碱型受体。我们比较了从吸烟者和非吸烟者分离出的PMN中[³H]尼古丁结合情况。吸烟者的[³H]尼古丁结合增加，并且与大脑情况一样，与烟草使用相关。淋巴细胞和PMN中烟碱型受体亚基的一个子集的mRNA和蛋白质表达表明，这些细胞类型同时含有α4β2和α3β4受体。
