Kiliç I, Kiliç B A, Güven C, Demirpençe E, Akşit M A
Department of Pediatrics, Pamukkale University Faculty of Medicine, Denizli, Turkey.
Biol Neonate. 2000 Oct;78(3):191-7. doi: 10.1159/000014270.
In order to investigate the role of nitric oxide (NO) in hypoxic tissue damage in newborns, we studied the effects of systemic administration of an inhibitor of NO synthase, N(G)-nitro-L-arginine (L-NNA), and the precursor for the synthesis of NO, L-arginine (L-ARG), on the biochemical and histological changes in brain, heart, lung, liver, kidney, intestine, and skeletal muscle tissues. Four groups of 1-day-old Wistar rat pups were used: control, hypoxic, L-ARG, and L-NNA groups. L-ARG 100 mg/kg or L-NNA 2 mg/kg was administered as a bolus intraperitoneally 1.5 h before hypoxia. Hypoxia increased lipid peroxidation in all tissues except muscle; this increase was prevented by L-NNA and L-ARG in brain, heart, lung, kidney, and liver tissues. L-NNA in intestine and L-ARG in muscle tissue increased lipid peroxidation. The tissue-associated myeloperoxidase activity was decreased in the liver by L-NNA and L-ARG. Histopathological changes in intestines were villous epithelial separation and hyperemia in hypoxic and L-NNA groups which were not observed in control and L-ARG groups. In lungs, pulmonary hemorrhage was observed only in the hypoxic group. These data suggest that NO acts both as a destructive and a protective agent in the pathogenesis of hypoxia-reoxygenation injuries.
为了研究一氧化氮（NO）在新生儿缺氧性组织损伤中的作用，我们研究了全身给予一氧化氮合酶抑制剂N(G)-硝基-L-精氨酸（L-NNA）以及一氧化氮合成前体L-精氨酸（L-ARG）对脑、心脏、肺、肝脏、肾脏、肠道和骨骼肌组织生化及组织学变化的影响。使用了四组1日龄的Wistar大鼠幼崽：对照组、缺氧组、L-ARG组和L-NNA组。在缺氧前1.5小时腹腔内推注给予100mg/kg的L-ARG或2mg/kg的L-NNA。缺氧增加了除肌肉外所有组织中的脂质过氧化；在脑、心脏、肺、肾脏和肝脏组织中，L-NNA和L-ARG可防止这种增加。肠道中的L-NNA和肌肉组织中的L-ARG增加了脂质过氧化。L-NNA和L-ARG降低了肝脏中与组织相关的髓过氧化物酶活性。肠道组织病理学变化在缺氧组和L-NNA组中表现为绒毛上皮分离和充血，而在对照组和L-ARG组中未观察到。在肺中，仅在缺氧组观察到肺出血。这些数据表明，在缺氧-复氧损伤的发病机制中，NO既起破坏作用又起保护作用。
