Pekkala E, Hietala E L, Puukka M, Larmas M
Institute of Dentistry, University of Oulu, Finland.
Acta Odontol Scand. 2000 Aug;58(4):155-9. doi: 10.1080/000163500429145.
Previous studies show that a high sucrose diet reduces the rate of primary dentinogenesis and increases dental caries, although their cause-effect relationship is still obscure. The purpose of this study was to explore whether the effect of sucrose load on the dentinogenesis and dental caries of young rat molars is mediated by systemic (intragastric) or by systemic and local (dietary) factors. At weaning (19 days), animals were randomized into the control, intragastric sucrose, and dietary sucrose groups for 4 weeks. The areas of dentin appositions and dentinal caries lesions were measured planimetrically. Caries was also determined with Shiffs staining and the width of predentin by histology. Urinary Ca, K, and Na levels were measured by flame photometry, urinary P levels using an UV method, and serum insulin levels using radioimmunoassay. Systemic and local sucrose load reduced dentin appositions and intragastric sucrose increased urinary Ca excretion. No differences in the width of predentin were noticed. Only dietary sucrose enhanced the occurrence and progression of caries. The present findings show that sucrose load reduces dentinogenesis by impairing the synthesis of dentin matrix, but also point out the crucial importance of the local sucrose challenge in the initiation of dental caries.
以往的研究表明，高蔗糖饮食会降低原发性牙本质生成的速率并增加龋齿，尽管它们之间的因果关系仍不明确。本研究的目的是探讨蔗糖负荷对幼鼠磨牙牙本质生成和龋齿的影响是由全身（胃内）因素介导，还是由全身和局部（饮食）因素共同介导。在断奶时（19天），将动物随机分为对照组、胃内蔗糖组和饮食蔗糖组，为期4周。通过平面测量法测量牙本质沉积面积和牙本质龋损面积。还通过希夫氏染色确定龋齿情况，并通过组织学测量前期牙本质的宽度。通过火焰光度法测量尿钙、钾和钠水平，用紫外法测量尿磷水平，用放射免疫分析法测量血清胰岛素水平。全身和局部蔗糖负荷均减少牙本质沉积，胃内蔗糖增加尿钙排泄。前期牙本质宽度未发现差异。只有饮食蔗糖会促进龋齿的发生和发展。目前的研究结果表明，蔗糖负荷通过损害牙本质基质的合成来降低牙本质生成，但也指出了局部蔗糖刺激在龋齿发生中的关键重要性。
