Rantonen P J, Meurman J H
Department of Clinical Chemistry, Kuopio University Hospital, Finland.
Acta Odontol Scand. 2000 Aug;58(4):160-5. doi: 10.1080/000163500429154.
The correlations between salivary proteins and the daytime variations are not known. The present study investigated the within-subject variation of correlations and concentrations between lysozyme, IgA, IgG, IgM, albumin, amylase, and total protein in stimulated whole saliva of healthy adults in the course of a 12-h period. After several practise sessions, unstimulated and stimulated whole saliva samples were collected five times daily (at 8 a.m., 11 a.m., 2 p.m., 5 p.m., and 8 p.m.) from 30 healthy university students. Flow rate and total protein concentration were used as covariates, and gender as a between-subject factor in the MANOVA analysis. After this adjustment, there was significant within-subject variation in salivary IgA (P < 0.001), albumin (P < 0.01), amylase (P < 0.05), and total protein (P < 0.001) concentrations. Total protein correlated significantly with amylase albumin and IgA through different samplings. In addition, IgG correlated with albumin and lysozyme in the course of 12 h. On the whole, the correlations between variables remained stable during repeated samplings. In addition, rankings of subjects for the variables tended to be maintained across different samplings (P < 0.001). However, the observed within-subject variations in salivary IgA, albumin, amylase, and total protein concentrations suggest that these proteins are subject to short-term variation.
唾液蛋白与日间变化之间的相关性尚不清楚。本研究调查了健康成年人在12小时内刺激全唾液中溶菌酶、IgA、IgG、IgM、白蛋白、淀粉酶和总蛋白之间的相关性及浓度的个体内变化。经过几次练习后，每天从30名健康大学生中收集五次未刺激和刺激后的全唾液样本（上午8点、上午11点、下午2点、下午5点和晚上8点）。流速和总蛋白浓度用作协变量，性别作为多变量方差分析中的个体间因素。经过这种调整后，唾液IgA（P < 0.001）、白蛋白（P < 0.01）、淀粉酶（P < 0.05）和总蛋白（P < 0.001）浓度存在显著的个体内变化。总蛋白通过不同采样与淀粉酶、白蛋白和IgA显著相关。此外，IgG在12小时内与白蛋白和溶菌酶相关。总体而言，变量之间的相关性在重复采样期间保持稳定。此外，不同采样中各变量的受试者排名倾向于保持一致（P < 0.001）。然而，观察到的唾液IgA、白蛋白、淀粉酶和总蛋白浓度的个体内变化表明，这些蛋白质存在短期变化。
