Zhu W, Vigh G
Texas A&M University, Department of Chemistry, College Station 77842-3012, USA.
J Chromatogr A. 2000 Sep 15;892(1-2):499-507. doi: 10.1016/s0021-9673(00)00387-3.
The newest member of the single-isomer isomer sulfated cyclodextrin family, octakis(2,3-diacetyl-6-sulfato)-gamma-cyclodextrin (ODAS-gamma-CD) was used for the first time as a resolving agent for the nonaqueous capillary electrophoretic separation of the enantiomers of 26 weak base pharmaceuticals in an acidic methanol background electrolyte. The solubility limit of ODAS-gamma-CD at room temperature proved to be 55 mM in this background electrolyte, which afforded good, fast enantiomer separations for most of the basic drugs tested. For all the bases studied, the effective mobilities and separation selectivities were found to follow the predictions of the charged resolving agent migration model of electrophoretic enantiomer separations. The effective mobilities of the weakly binding weak bases remained cationic throughout the entire 0 to 45 mM ODAS-gamma-CD concentration range; separation selectivities increased as the ODAS-gamma-CD concentration was increased. The effective mobilities of the moderately binding weak bases became anionic in the 2.5 to 45 mM ODAS-gamma-CD concentration range; separation selectivities first increased as the effective mobilities approached zero, then decreased again as the ODAS-gamma-CD concentration was increased further. The effective mobilities of the strongly binding weak bases became anionic in the 0 to 2.5 mM ODAS-gamma-CD concentration range; separation selectivities decreased as the ODAS-gamma-CD concentration was increased above 2.5 mM.
单异构体硫酸化环糊精家族的最新成员，八（2,3 - 二乙酰基 - 6 - 硫酸根）-γ-环糊精（ODAS - γ-CD）首次被用作拆分剂，用于在酸性甲醇背景电解质中对26种弱碱性药物对映体进行非水毛细管电泳分离。在该背景电解质中，室温下ODAS - γ-CD的溶解度极限证明为55 mM，这为大多数测试的碱性药物提供了良好、快速的对映体分离效果。对于所有研究的碱，发现有效迁移率和分离选择性符合电泳对映体分离的带电拆分剂迁移模型的预测。在整个0至45 mM ODAS - γ-CD浓度范围内，弱结合弱碱的有效迁移率始终保持阳离子性；分离选择性随着ODAS - γ-CD浓度的增加而增加。在2.5至45 mM ODAS - γ-CD浓度范围内，中等结合弱碱的有效迁移率变为阴离子性；分离选择性首先随着有效迁移率接近零而增加，然后随着ODAS - γ-CD浓度进一步增加而再次降低。在0至2.5 mM ODAS - γ-CD浓度范围内，强结合弱碱的有效迁移率变为阴离子性；当ODAS - γ-CD浓度增加到2.5 mM以上时，分离选择性降低。
