Varon M, Seijffers J
J Bacteriol. 1975 Dec;124(3):1191-7. doi: 10.1128/jb.124.3.1191-1197.1975.
Symbiosis-independent (Sin) mutants were isolated from the symbiosis-dependent and symbiosis-competent (Sdcomp+) Bdellovibrio bacteriovorus 109J. Independently isolated Sin mutants were examined for their symbiosis competence and most were found to be comp+. Bdellovibrios comp- were selected from the Sincomp+ mutants. The Sincomp+ bdellovibrios are always at a selective disadvantage, either against Sincomp- bdellovibrios (in organic medium) or against Sdcomp+ bdellovibrios (in buffer with Escherichia coli cells).
从依赖共生且具备共生能力(Sdcomp+)的蛭弧菌109J中分离出非共生(Sin)突变体。对独立分离出的Sin突变体进行共生能力检测,发现大多数为comp+。从Sincomp+突变体中筛选出comp-的蛭弧菌。Sincomp+蛭弧菌总是处于选择劣势,在有机培养基中相对于Sincomp-蛭弧菌,或在含有大肠杆菌细胞的缓冲液中相对于Sdcomp+蛭弧菌。